ELISpot will refine our understanding of the T cell response to AAV, which could aid in improvements to vector design, patient safety, and treatment efficacy. cell ELISpot Assay
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Monitoring Immune Responses in Gene Therapy Clinical Trials Using ELISpot
Use of the validated IFN-γ ELISpot method in a clinical trial of AAV5-FVIII gene therapy demonstrated the utility of the assay for monitoring immune responses to viral vector capsids and expressed …
Assessing Antigen-Specific T Cell Responses Through IFN-γ ... - Springer
Apr 16, 2024 · Here, we optimize and describe, in a step-by-step fashion, how to perform a controlled IFN-γ ELISpot experiment to determine the frequency of responding or antigen-specific T cells in …
Aside from the viral genome, the capsid of AAV serotype 2 (AAV2) may also interact with the innate immune system via TLR2, recently shown to occur in nonparenchymal cells (Kupffer and...
Immunoassays for immunotherapy research - Mabtech
Feb 23, 2024 · ELISpot and FluoroSpot are perfect potency assays to use during the development of immunotherapies as well as cell and gene therapies, offering critical insights into CAR-T cell …
Understanding and Tackling Immune Responses to Adeno-Associated …
Sep 19, 2023 · Helper cells will amplify and expand both the cellular and humoral immune response (Fig. 1) and increase the anti-AAV antibody secretion by B cells. 108 Several assays have been …
ELISpot assays: Pioneering immunogenicity test methods
Nov 14, 2023 · As potential vaccine candidates are evaluated, ELISpot assays assist researchers in gauging the efficacy of these vaccines by quantitatively measuring the immune responses they invoke.
An overview of the challenges and implementation of ELISpots: an ...
Jun 28, 2023 · In this interview, Michelle Miller (BioAgilytix; NC, USA) provides a basic overview of an ELISpot assay and how the method is used for the safety testing of therapeutics.
Immune responses to AAV vectors: overcoming barriers to successful …
Jul 4, 2013 · Small-scale clinical studies have enabled investigators to dissect the immune responses to the AAV vector capsid and to the transgene product, and to develop strategies to manage these …